Course: Physiology and Molecular Biology of Animal Cells

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Course title Physiology and Molecular Biology of Animal Cells
Course code KBI/N016
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Semester Winter and summer
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory, Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Fišar Zdeněk, prof. RNDr. CSc.
  • Mareš Vladislav, doc. MUDr. DrSc.
Course content
Lectures: 1. Molecular and functional properties of cell membranes (composition, structure, semipermeability, fluidity, rafts, liposomes). 2. Membrane and vesicular transport in a cell (transport of water, ions, small organic molecules, ion channels, membrane potential, action potential, vesicles, molecular motors, endocytosis). 3. Biologicaly active substances (biological signals, signal transmission and cellular response, receptors, enzymes, hormones, cytokines, growth factors, neurotransmitters). 4. Cell-signalling (signal transduction, intracellular signalling pathways and responses). 5. Cytoskeleton (morphology, adhesion, movement, intracellular transport, genome organisation, and cell division). 6. Cellular energetics (metabolism, redox reactions, glycolysis, mitochondria, Krebs cycle, respiratory chain, neuroplasticity, neurotoxicity). 7. Mechanisms of cell damage and reparation (free radicals, oxidative stress, antioxidative protection, hypoxia, ischemia). 8. Nucleus and regulation of gene expression (RNA replication, modifications and types of RNA, translation, regulation of transcription, differentiation, stem cells). 9. Protein synthesis and modification. 10. Cell death. 11. Cell phenotypes. 12. Tissues (cell-cell junctions, extracellular matrix). 13. Neuro-immune-endocrine regulation; senescence; neurodegeneration. Practices: 1. Cell models (isolation of cels, membranes and organels; liposomes; centrifugation). 2. Modelling of membrane potential - PC-based simulation using NeuroLab programme (resting potential - effect of ion balance and membrane permeability, velocity of action potential propagation). 3. Modelling of action potential - PC-based simulation using Neuromuscular Junction (NMJ) programme (effect of ions and blockators of ion channels on action potential). 4. Modelling of action potential - PC-based simulation using SimNerve programme (conditions for firing of action potentials and refractory period). 5. Virtual laboratory equipment for simulation of electrophysiological functions - PC-based simulation of patch clamp method using SimPatch programme (study of voltage-dependent ion channels in the neuronal membrane, measurement of ion currents and effects of blockators of ion channels). 6. Data analysis from electrophysiological experiments. 7. Experimental methods of cell biology 1: Fluorescence ion biological research. 8. Experimental methods of cell biology 2: Flow cytometry. 9. Experimental methods of cell biology 3: Respirometry. 10. Experimental methods of cell biology 4: Enzyme activities. 11. Experimental methods of cell biology 5: Radiochemical methods.

Learning activities and teaching methods
unspecified, unspecified, unspecified
Learning outcomes
The aim of the course is to provide deeper information about general and the phenotype specific structures and functions of animal cells under physiological and stress conditions.

Prerequisites
unspecified

Assessment methods and criteria
unspecified
Recommended literature
  • Alberts, B. a kol. Základy buněčné biologie.. Espero Publishing, Ústí nad Labem., 1998.
  • Fišar Z. a kol. Vybrané kapitoly z biologické psychiatrie, 2. vyd.. Grada, Praha, 2009.
  • Greenspan F.S., Baxter J.D. Základní a klinická endokrinologie. Praha, 2003.
  • Hořejší V., Bartůňková J. Základy imunologie. Triton, Praha, 2009.
  • Lodish H. et al. Molecular Cell Biology, W.H.Freeman and Co., New York, 2000-2012. 2012. ISBN 978-1429234139.
  • Lüllmann H. et al. Farmakologie a toxikologie. Grada, Praha, 2004.
  • Murray R.K., Bender D.A., Botham K.M., Kennelly P.J., Rodwell V.W., Weil P.A. Harperova ilustrovaná biochemie. Galén, Praha 2012..
  • Nichols J.G. a kol. Od neuronu k mozku.. Praha, 2013. ISBN 978-80-200-2155-7.
  • Schreiber V. Hormony a lidská mysl. Triton, Praha, 2004.
  • Siegel G.J. Basic Neurochemistry. Molecular, Cellular and Medical Aspects. Philadelphia, Lippincott-Raven, 1999. ISBN 0-397-51820-X.
  • Stahl S.M. Essential psychopharmacology: neuroscientific basis and practical applications, Cambridge Univ., 2000..
  • Štípek a kol. Antioxidanty a volné radikály ve zdraví a v nemoci. Avicenum, Grada, Praha, 2000.
  • Voet D., Voet J.G. Biochemie, 1995.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Biology (A12) Biology courses 1 Winter
Faculty of Science Biology (A16) Biology courses 1 Winter
Faculty of Science Chemistry (A14) Chemistry courses - -
Faculty of Science Toxicology and Hazardous Waste Analysis (A12) Chemistry courses - -
Faculty of Science Toxicology and Hazardous Waste Analysis (A14) Chemistry courses - -