Course: Methods of Molecular Biology II

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Course title Methods of Molecular Biology II
Course code KBI/N011
Organizational form of instruction Lesson
Level of course not specified
Year of study not specified
Semester Winter and summer
Number of ECTS credits 2
Language of instruction Czech
Status of course unspecified
Form of instruction unspecified
Work placements unspecified
Recommended optional programme components None
  • Štofik Marcel, Mgr. Ph.D.
Course content
1. Cloning of genomic DNA fragment into plasmid DNA, transformation of E. Coli - Restriction of plasmid DNA and electrophoresis of fragments, ligation with isolated fragment of DNA, preparation of competent cells of E. Coli, transformation of bacterial cells, identification of recombinant colonies, isolation of plasmid and restriction analysis. 2. Electrophoresis of proteins in the polyacrylamide gel (SDS-PAGE) 3. Detection of proteins by immunoblotting methods 4. Marking of nucleic acids, hybridization with fluorescent probes

Learning activities and teaching methods
Learning outcomes
The main goal of the course is to provide students basic skils in advanced methods in molecular biology. Students will practice isolation, purification, characterization and hybridization of DNA and chosen PCR techniques.


Assessment methods and criteria
Recommended literature
  • Bruce A. a kol. Základy buněčné biologie - Úvod do molekulární biologie buňky. Espero Publishing, Ústí nad Labem,. 2001.
  • Curell B.F. et al. Techniques for Engineering Genes. University of Greenwich, London. 1994.
  • Hubáček J. a kol. Vybrané kapitoly z molekulární genetiky mikroorganismů. Academia, Praha,. 1992.
  • Kaprálek, F. Základy bakteriologie. Karolinum, Praha. 1999.
  • Rosypal, S. a kol. Molekulární genetika. SPN, Praha,. 1989.
  • Rosypal S. a kol. Úvod do molekulární biologie I.-III. Academia, Praha. 2000.
  • Ruml T. Laboratoře z genového inženýrství. VŠCHT. 1997.
  • Vodrážka, Z. Biotechnologie. Academia, Praha,. 1992.
  • Vondrejs V. Genové inženýrství I a II. Karolinum, Praha. 2001.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester