Course: Methods of Molecular Biology I

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Course title Methods of Molecular Biology I
Course code KBI/N010
Organizational form of instruction Lecture
Level of course not specified
Year of study not specified
Semester Winter and summer
Number of ECTS credits 2
Language of instruction Czech
Status of course unspecified
Form of instruction unspecified
Work placements unspecified
Recommended optional programme components None
  • Malý Jan, Mgr. Ph.D.
Course content
1. Isolation of DNA and RNA 2. Electrophoresis and its variations 3. Work with enzymes 4. Recombinant DNA 5. Vectors, protein expression 6. Mutagenesis of DNA 7. Transformation of organisms, construction of RNA and DNA probes 8. Hybridization of nucleic acid 9. Southern, Northern and Western blotting 10. Principle and variations of polymerase chain reaction (PCR) 11. In vitro mutagenesis, interaction of DNA and proteins 12. Microsatelite DNA 13. DNA fingerprinting 14. RFLP and RAPD methods

Learning activities and teaching methods
Learning outcomes
The main goal of the course is to provide students theoretical basis of methods widely used in molecular biology research and genetic engineering.


Assessment methods and criteria
Recommended literature
  • Bruce A. a kol. Základy buněčné biologie - Úvod do molekulární biologie buňky. Espero Publishing, Ústí nad Labem,. 2001.
  • Curell B.F. et al. Techniques for Engineering Genes. University of Greenwich, London. 1994.
  • Hubáček J. a kol. Vybrané kapitoly z molekulární genetiky mikroorganismů. Academia, Praha,. 1992.
  • Kaprálek, F. Základy bakteriologie. UK Karolinum, Praha,. 2000.
  • Rosypal, S. a kol. Molekulární genetika. SPN, Praha,. 1989.
  • Rosypal S. a kol. Úvod do molekulární biologie I.-III. Academia, Praha. 2000.
  • Ruml T. Laboratoře z genového inženýrství. VŠCHT. 1997.
  • Vodrážka, Z. Biotechnologie. Academia, Praha,. 1992.
  • Vondrejs V. Genové inženýrství l, UK Karolinum, Praha. 1997.
  • Vondrejs V. Genové inženýrství ll, UK Karolinum, Praha. 2001.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester