Course: Selected Topics in Genetics and Molecular Biology

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Course title Selected Topics in Genetics and Molecular Biology
Course code KBI/0067
Organizational form of instruction Lecture
Level of course not specified
Year of study not specified
Semester -
Number of ECTS credits 2
Language of instruction Czech
Status of course unspecified
Form of instruction unspecified
Work placements unspecified
Recommended optional programme components None
  • Ipser Jan, RNDr. CSc.
Course content
1. Isolation and purification of nucleic acids. 2. Centrifugation methods. 3. Electrophoretic separation of nucleic acids. 4. Electrophoretic separation of proteins. 5. Blotting methods - DNA, RNA, proteins. 6. PCR. 7. DNA profiling. 8. DNA sequencing. 9. cDNA preparation. 10. Cloning. Gene and genetic library. 11. Methods of transgenosis. GMO. 12. Molecular biology of plasmids. 13. Essential genomics. 14. Essential proteomics.

Learning activities and teaching methods
Learning outcomes
The aim of the course is to provide information on modern genetic, biochemical, biophysical and molecular biology methods (such as various types od centrifugation, electrophoresis, EIA, ELISA, RIA, autoradiography, PCR, nucleic acid sequencing, finger printing, VNTR, RFLP, RAPD, blotting, genetic mapping and cloning, transgenosis). Laboratory exercising of the selected methods is included, too. Genetics or Molecular biology or Biology of Procaryotes and Viruses or Cellular Principles of Life Processes or Cellular and General Biology.


Assessment methods and criteria
Recommended literature
  • Nečas O. a kol. Obecná biologie. 2000.
  • Nečásek J. a kol. Obecná genetika.. 1979.
  • Rosypal S. a kol. Nový přehled biologie.. 2003.
  • Rosypal S., Doškař J. Úvod do molekulární biologie I-III.. 1997.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester